Trophectoderm biopsy, what is it?

The trophectoderm or trophoblast – is presented by the cells of an embryo at the stage of “blastocyst” development. This is usually the 5th day of embryo development, but sometimes the 6th or even the 7th.

Embryo biopsy is performed by an experienced embryologist using specific laboratory equipment (laser hatching and micromanipulators). A biopsy is performed in high-quality embryos, at the stage of cell division, when it contains 156-200 cells. In fact, we need no more than 5-6 cells for diagnosis, and it is absolutely safe for the embryo.
Embryo biopsy is necessary for preimplantation genetic testing to rule out chromosomal and monogenic disorders. The only transfer of a chromosomally/genetically healthy embryo makes it possible to increase the probability of implantation (attachment of the embryo) and the birth of a healthy child, and reduces the duration of infertility treatment.

Cells obtained as a result of a biopsy can be examined by various methods: FISH, PCR, CGH and NGS. The last method, NGS, we use to test embryos that were grown in our laboratory. The choice of the method of preimplantation genetic testing to rule out aneuploidy (chromosomal disorders) PGT-A (PGT-A) or monogenic diseases PGT-M (PGT-M) is prescribed individually, depending on the indications for each couple.

After the biopsy of the trophectoderm, the embryos are vitrified (frozen), each on a separate cryocarrier and stored in a cryogenic storage dewars in liquid nitrogen until the test results are obtained. Based on the obtained results, the strongest healthy embryo is selected, which is transferred to the uterine cavity after preparation for embryo transfer.

Biopsy of trophectoderm cells is a reliable and proven modern method to define aneuploidy or monogenic disorders at embryo estimation, which increases the occurrence and carrying of pregnancies. The birth of a healthy child and the happiness of your family is contributed by this method.

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